Sindbis Virus Maturation
نویسندگان
چکیده
منابع مشابه
Genome of Sindbis virus.
(32)P-labeled ribonucleic acid (RNA) from purified Sindbis virus was examined for the presence of hidden breaks. Viral RNA was treated with acid at pH 2.9 or with formaldehyde and was analyzed on sucrose gradients or by polyacrylamide gel electrophoresis. The sedimentation pattern and mobility on polyacrylamide gels of the 42S RNA was unaffected by heating and quick cooling and was not altered ...
متن کاملPhosphorylated proteins of Sindbis virus.
The capsid and two membrane proteins of Sindbis virus, grown in chicken cells, contain 0.03 to 0.1 mol of phosphate per mol of protein.
متن کاملAgglutination of Sindbis virus and of cells infected with Sindbis virus by plant lectins.
We have examined the agglutination of Sindbis virus and of chick and hamster cells infected with Sindbis virus by two of the plant lectins, concanavalin A and Ricinus communis agglutinin. Both lectins agglutinate the virus by binding to the polysaccharide chains of the envelope glycoproteins. Both chick and hamster cells exhibit increased agglutination by the lectins after infection by Sindbis ...
متن کاملEnzymatic iodination of Sindbis virus proteins.
Sindbis virus was iodinated by using the enzyme lactoperoxidase, an iodination technique which labels only surface proteins. By this technique, the two viral glycoproteins are labeled, and the internal viral protein is not. The two glycoproteins are iodinated to strikingly different extents. This difference in susceptibility to iodination apparently is due to the position or conformation of the...
متن کاملHexagonal glycoprotein arrays from Sindbis virus membranes.
Freeze-etch electron microscopy of Sindbis virus and of glycoprotein arrays derived from Sindbis membranes by nonionic detergent treatment shows that the local geometry of glycoprotein-glycoprotein interaction does not depend on the presence of the nucleocapsid.
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ژورنال
عنوان ژورنال: Journal of General Virology
سال: 1973
ISSN: 0022-1317,1465-2099
DOI: 10.1099/0022-1317-18-3-375